| 质粒名称: | HA-FOXO3a WT-ER |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 8355 |
| 存储实验室: | Michael Greenberg |
|
载体骨架基本信息 |
|
| 载体名称: | pECE |
| 载体出品公司: | Clontech |
| 空载体大小: | 2900 bp |
| 完整质粒大小: | 5946 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | FOXO3a、FKHRL1、forkhead family |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3000bp |
| 基因突变: | mouse estrogen receptor (G525R) on C-term |
| 标签/融合蛋白: | HA (YDVPDYASL) (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | HindIII (not destroyed) |
| 3’克隆位点: | XbaI (not destroyed) |
| 5’测序引物 | SV40pro-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | HA-FOXO3a WT-ER was a gift from Michael Greenberg (Addgene plasmid # 8355 ; http://n2t.net/addgene:8355 ; RRID:Addgene_8355) |
| 参考文献部分: | DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS, Chiang LW, Greenberg ME. Science 2002 Apr 19;296(5567):530-4. 10.1126/science.1068712 PubMed 11964479 |
买家导航
HA-FOXO3a WT-ER载体质粒基本信息
HA-FOXO3a WT-ER质粒图谱载体图谱和HA-FOXO3a WT-ER载体序列质粒序列多克隆位点信息
HA-FOXO3a WT-ER质粒载体简介
HA-FOXO3a WT-ER质粒序列载体序列
LOCUS HA-FOXO3a WT-ER 5946 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5946)
AUTHORS Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS,
Chiang LW, Greenberg ME
TITLE DNA repair pathway stimulated by the forkhead transcription factor
FOXO3a through the Gadd45 protein.
JOURNAL Science 2002 Apr 19;296(5567):530-4.
PUBMED 11964479
REFERENCE 2 (bases 1 to 5946)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Science";
date: "2002-04-19"; volume: "296(5567)"; pages: "530-4"
COMMENT Sequence Label: HA-FOXO3a WT-ER
FEATURES Location/Qualifiers
source 1..5946
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 115..250
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(153..172)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 207..226
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(309..328)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(587..604)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(758..1346)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(838..857)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(1518..2309,2310..2378))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2141..2160
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2379..2483)
/gene="bla"
/label=AmpR promoter
promoter 2513..2870
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2513..2704
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2536..2556)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2721..2856
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2895..2921
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/note="This is a variant of the standard HA tag, which has
sequence YPYDVPDYA. Some anti-HA antibodies may not
recognize this variant."
/translation="YDVPDYASL"
primer_bind 4958..4974
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4958..4974
/label=pBluescriptSK
/note="For pBluescript vector"
ORIGIN
1 taagtaatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
121 tttattgcag gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
241 tgtctggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
301 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
361 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
421 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
481 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtgcggtgt gaaataccgc
541 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
1021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
1081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
1141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
1201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
1261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
1321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
1381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
1441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaaag tatatatgag
1501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
1561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
1621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
1681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
1741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
1801 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
1861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
1921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
1981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2101 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
2161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
2281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
2341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
2401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
2461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ttctgaggcg
2521 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
2641 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
2701 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
2761 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
2821 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa agatctaagc
2881 ttgccgccac catgtatgat gttcctgatt atgctagcct cccggggaat tcggcagagg
2941 caccggcttc cccggccccg ctctctccgc tcgaagtgga gctggacccg gagttcgagc
3001 cccagagccg tccgcgatcc tgtacgtggc ccctgcaaag gccggagctc caagcgagcc
3061 ctgccaagcc ctcgggggag acggccgctg actccatgat ccccgaggag gaggacgatg
3121 aagacgacga ggacggcggg ggacgggccg gctcggccat ggcgatcggc ggcggcggcg
3181 ggagcggcac gctgggctcc gggctgctcc ttgaggactc ggcccgggtg ctggcacccg
3241 gagggcaaga ccccgggtct gggccagcca ccgcggcggg cgggctgagc gggggtacac
3301 aggcgctgct gcagcctcag caaccgctgc caccgccgca gccgggggcg gctgggggct
3361 ccgggcagcc gaggaaatgt tcgtcgcggc ggaacgcctg gggaaacctg tcctacgcgg
3421 acctgatcac ccgcgccatc gagagctccc cggacaaacg gctcactctg tcccagatct
3481 acgagtggat ggtgcgttgc gtgccctact tcaaggataa gggcgacagc aacagctctg
3541 ccggctggaa gaactccatc cggcacaacc tgtcactgca tagtcgattc atgcgggtcc
3601 agaatgaggg aactggcaag agctcttggt ggatcatcaa ccctgatggg gggaagagcg
3661 gaaaagcccc ccggcggcgg gctgtctcca tggacaatag caacaagtat accaagagcc
3721 gtggccgcgc agccaagaag aaggcagccc tgcagacagc ccccgaatca gctgacgaca
3781 gtccctccca gctctccaag tggcctggca gccccacgtc acgcagcagt gatgagctgg
3841 atgcgtggac ggacttccgt tcacgcacca attctaacgc cagcacagtc agtggccgcc
3901 tgtcgcccat catggcaagc acagagttgg atgaagtcca ggacgatgat gcgcctctct
3961 cgcccatgct ctacagcagc tcagccagcc tgtcaccttc agtaagcaag ccgtgcacgg
4021 tggaactgcc acggctgact gatatggcag gcaccatgaa tctgaatgat gggctgactg
4081 aaaacctcat ggacgacctg ctggataaca tcacgctccc gccatcccag ccatcgccca
4141 ctgggggact catgcagcgg agctctagct tcccgtatac caccaagggc tcgggcctgg
4201 gctccccaac cagctccttt aacagcacgg tgttcggacc ttcatctctg aactccctac
4261 gccagtctcc catgcagacc atccaagaga acaagccagc taccttctct tccatgtcac
4321 actatggtaa ccagacactc caggacctgc tcacttcgga ctcacttagc cacagcgatg
4381 tcatgatgac acagtcggac cccttgatgt ctcaggccag caccgctgtg tctgcccaga
4441 attcccgccg gaacgtgatg cttcgcaatg atccgatgat gtcctttgct gcccagccta
4501 accagggaag tttggtcaat cagaacttgc tccaccacca gcaccaaacc cagggcgctc
4561 ttggtggcag ccgtgccttg tcgaattctg tcagcaacat gggcttgagt gagtccagca
4621 gccttgggtc agccaaacac cagcagcagt ctcctgtcag ccagtctatg caaaccctct
4681 cggactctct ctcaggctcc tccttgtact caactagtgc aaacctgccc gtcatgggcc
4741 atgagaagtt ccccagcgac ttggacctgg acatgttcaa tgggagcttg gaatgtgaca
4801 tggagtccat tatccgtagt gaactcatgg atgctgatgg gttggatttt aactttgatt
4861 ccctcatctc cacacagaat gttgttggtt tgaacgtggg gaacttcact ggtgctaagc
4921 aggcctcatc tcagagctgg gtgccaggcg cggccgctct agaactagtg gatccacgaa
4981 atgaaatggg tgcttcagga gacatgaggg ctgccaacct ttggccaagc cctcttgtga
5041 ttaagcacac taagaagaat agccctgcct tgtccttgac agctgaccag atggtcagtg
5101 ccttgttgga tgctgaaccg cccatgatct attctgaata tgatccttct agacccttca
5161 gtgaagcctc aatgatgggc ttattgacca acctagcaga tagggagctg gttcatatga
5221 tcaactgggc aaagagagtg ccaggctttg gggacttgaa tctccatgat caggtccacc
5281 ttctcgagtg tgcctggctg gagattctga tgattggtct cgtctggcgc tccatggaac
5341 acccggggaa gctcctgttt gctcctaact tgctcctgga caggaatcaa ggtaaatgtg
5401 tggaaggcat ggtggagatc tttgacatgt tgcttgctac gtcaagtcgg tcccgcatga
5461 tgaacctgca gggtgaagag tttgtgtgcc tcaaatccat cattttgctt aattccggag
5521 tgtacacgtt tctgtccagc accttgaagt ctctggaaga gaaggaccac atccaccgtg
5581 tcctggacaa gatcacagac actttgatcc acctgatggc caaagctggc ctgactctgc
5641 agcagcagca tcgccgccta gctcagctcc ttctcattct ttcccatatc cggcacatga
5701 gtaacaaacg catggagcat ctctacaaca tgaaatgcaa gaacgtggta cccctctatg
5761 acctgctcct ggagatgttg gatgcccacc gccttcatgc cccagccagt cgcatgggag
5821 tgcccccaga ggagcccagc cagacccagc tggccaccac cagctccact tcagcacatt
5881 ccttacaaac ctactacata cccccggaag cagagggctt ccccaacacg atctgagagc
5941 tctaga
//
